Contact Information

100 Radcliffe Drive, Chestertown, MD 21620, USA

Toll Free: 866-ILS-1199

Local: (410) 810-7506

Fax: (410) 810-7508

Member Of

Laboratory Services

  • 1

    TISSUE SECTIONING

    Sections can be taken from FFPE blocks or frozen tissues, utilizing banked or custom collected specimens. Frozen tissues must first be embedded in OCT prior to sectioning. Sections can be placed on plus slides or into microcentrifugetubes. DNA projects require a blade change and stage cleaning procedure between samples. Basic chemical stains such as H+E are available. A minimum of 5 slides per block are required if only slides are ordered. This requirement will be waived if the FFPE of frozen tissue will also be purchased.

    Paraffin Sections:
      Unstained $20.00
      H&E $25.00
      Curls $20.00
    Frozen Sections:
      Unstained $30.00
      H&E $35.00
      Curls $30.00
      OCT Embedding Only $20.00

    DNA projects requiring a blade change between samples will be charged an additional $3.00 per block.

    A minimum of 5 slides per block are required if only slides are ordered. This requirement will be waived if the FFPE of frozen tissue will also be purchased.

  • 2

    CUSTOM TISSUE EXTRACTIONS

    DNA, RNA, mRNA, and Protein can be extracted from banked or custom collected frozen tissues. RNA is extracted using the standard TRIZOL protocol. DNA is extracted using TRIZOL or a proprietary protocol. Custom protocols can be utilized upon request. Currently available DNA and DNA samples can be purchased online.

    All protein work is handled by ILSbio’s sister company, Protein Biotechnologies (San Diego, CA). Protein Lysates are generated using a Modified RIPA Buffer protocol. Many Lysates are currently available or can be custom produced from ILSbio’s tissues. Protein Biotechnologies also offers a wide range of easy to use pre-made protein dipstick assays. Called Dip-N-Blot and Dip-N-Spot Protein Assay Strips, these strips eliminate a researcher’s requirement for specialized equipment and empower them to generate and analyze experimental data quickly.

    RNA or DNA from ILSBio Tissues - 10ug aliquots: (Includes cost of tissue and all laboratory fees)
    Gen Oncology Tumor $180.00
      Gen Oncology NAT $150.00
      Rare Oncology Tumor $280.00
      Rare Oncology NAT $230.00
      Normal (Surgical) $230.00
      Normal (Post Mortem) $230.00
    RNA or DNA from your tissues:
      Extraction $165.00 per sample
      QC on Agilent 2100 $120.00 per 12 samples
    Protein
    All protein work is performed by our partner, Protein Biotechnologies. Inquire for pricing.
  • 3

    TISSUE MICROARRAYS

    Manufacture of Custom Tissue Microarrays (TMA) can be performed using ILSbio's high-quality tissues. Standard configuration for a Tumor Matched Pair TMA consists of two tumor cores and one NAT core for each case. Duplication of the tumor core prevents drop out due to layers of fibrous, normal, or necrotic tissue in the sample, ensuring every section is representative of the tumor. Custom configurations can be performed as well. All tissues to be used for a TMA are prescreened using a top slide to identify the areas of the highest tumor cell percentage.

    Tissue microarrays allow a researcher to process and analyze all of their samples at one time. This greatly accelerates the time to results while reducing the reagents needed to run an assay. The short time frame to results also reduces inter-assay or inter-observer variability.

    Manufacture of Custom TMA using ILSbio's high-quality tissues. Standard configuration for a Tumor Matched Pair TMA consists of two tumor cores and one NAT core for each case. Duplication of the tumor core prevents drop out due to layers of fibrous, normal, or necrotic tissue in the sample, ensuring every section is representative of the tumor. Custom configurations can be performed as well. All tissues to be used for a TMA are prescreened using a top slide to identify the areas of the highest tumor cell percentage.

    Paraffin:
      Construction Set Up $250.00
      Core Transfer $10.00 per core
    Frozen:
      Construction Set Up $350.00
      Core Transfer $20.00 per core
      OCT Embedding and Top Slide $50.00

    Pricing does not include tissue costs.

  • 4

    Extraction Microarrays

    SomaPlex Reverse Phase ProteinMicroarrays are currently available from Protein Biotechnologies. DNA and RNA Microarrays are made by request from ILSbio’s well-characterized samples.

    Microarrays are protein lysates or purified RNA or DNA from dozens, hundreds or thousands of clinical specimens printed or spotted onto specialized microscope slides. The spots are extremely small (~0.1 - 0.2 mm) so a large number can be printed on a single slide. Using specific labeled molecular probes, the researcher can interrogate the slide for the presence or absence of a specific biomarker generating a vast amount of data within a few hours. These products address the need for high throughput biomarker screening formats that are increasingly necessary for novel diagnostic test and drug development. Additionally, they reduce the amount of research reagents necessary to obtain a result relative to more conventional analytic techniques thus reducing costs to the end-user.

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